Note: This product contains powder, NOT liquid. When fully dissolved in distilled water, it makes a total of 10 liters. The formula concentration is 5.36 g per liter.
Please order the Product Express post and put the product in the fridge as soon as it arrives. Do not put in the Freezer.
Expiration Standard: 3 Years from the manufacture date.
Storage Conditions: Store in the fridge at 2°-8°C, avoid direct light.
Supplied in powdered form — when mixed with water, it makes the volume you’ve selected.
DKW Medium – Driver and Kuniyuki Walnut Medium
DKW Medium, short for Driver and Kuniyuki Walnut Medium, was initially formulated for the micropropagation of walnut using nodal explants. Today, it is widely used across various plant species due to its unique nutrient profile—characterized by lower nitrogen and higher sulfate concentrations—which may positively influence shoot regeneration and overall plant development.
Key Benefits
Convenient and straightforward to use
Supplies essential nutrients for optimal in vitro plant growth
Formulated with reduced nitrogen and elevated sulphate levels
Supports multiple shoot induction
Enables callus-mediated shoot regeneration
How to Prepare DKW Medium (1 Litre)
Follow these steps for reliable media preparation:
In a beaker, pour 800 mL of distilled water.
Add the following:
5.36 grams of PCT’s DKW Medium powder
30 grams of sugar
1–2 mL of PPM™ (Plant Preservative Mixture)
Top up the solution to a final volume of 1000 mL.
Adjust the pH to 5.4–5.8 using a pH meter and appropriate reagents.
Incorporate a gelling agent:
6–8 g/L agar or 3–4 g/L gellan gum, and continue stirring.
Autoclave the medium at 121°C and 15 psi for 20 minutes.
Your DKW medium is now ready for use!
This Products is shipped from Marrickville, Sydney.
Shipping Cost and Time—The shipping cost will be displayed on the checkout screen.
Handling Time—Packages typically takes 0-3 days to prepare for shipment. Unless otherwise stated as ‘Lead time’ on the listing as something different.
Please note that handling time may vary, especially during busy holidays or sale period.
Certain products, like Colonised agar plates or Sawdust spawn, Spray and grow kit, have longer handling times as they are made to order.
Available on Request
-Multiple shoot induction
-Callus-mediated shoot regeneration
-Improved success rates for specific plant species (e.g., turmeric and walnut)
-Used to tissue culture woody plants
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