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Home / Mushroom Spawn / Shiitake Mushroom Spawn (3782) | Lentinula edodes
A hand holds a large cluster of light brown Lentinula edodes from the Shiitake – 3782 Mushroom Grow Kit (Spray and Grow, 3kg) outdoors, with grass in the background.
A plastic bag filled with grain colonised by white mushroom mycelium against a white background.
A sealed plastic bag filled with small, round, light brown grains on a white background.
A sealed plastic bag filled with white mycelium and grain on a white background. A cluster of brown shiitake mushrooms (Lentinula edodes) grows from a white substrate, cultivated with Shiitake Mushroom Spawn (3782) | Lentinula edodes, against a plain white background.
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Australian Made
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Shiitake Mushroom Spawn (3782) | Lentinula edodes

Price range: $15.00 through $40.00 inc. GST
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Category Mushroom Spawn, Shiitake Mushrooms
Brand Rootlab

Product Details

Species Information

Common name
Shiitake – 3782
Scientific name
Lentinula edodes
Incubation temperature
21°C - 27°C
Fruiting temperature
7°C - 25°C
Fruiting humidity
≥ 75%
CO2 requirement (PPM)
< 600 for pinning and then < 1200 for fruit development, keep below < 1500 to avoid leggy stem and small cap ppm
Preferred substrate
80% hardwood sawdust + 20% bran
Fruiting orientation
Top
Storage
Store between 2°C-4°C in fridge, do not freeze
Difficulty
Intermediate
Notes
Shiitakes are grown on a mix of sawdust and bran, generally 80% sawdust and 20% bran or 90% sawdust and 10% bran. Do not grow on master's mix, as it is highly likely to be contaminated. Shiitake mushrooms (Lentinula edodes) have a unique cultivation process compared to many other mushroom species. While traditionally grown on logs, they can also be successfully cultivated on 3-4 kg substrate bags of sawdust. The mycelium colonization for shiitake is notably slow, taking around 6 weeks for complete colonization. During this process, the mycelium initially appears white but then undergoes a distinctive browning phase, which takes about two additional weeks. Once fully colonized, the plastic bag(mushroom bag) is removed, and the entire block is placed in a fruiting room with high humidity (80-90%) and temperatures between 7-21°C. After the first flush of mushrooms is harvested, allowing the block to rest for about two weeks before initiating the next fruiting cycle is crucial. This resting period is essential to prevent contamination. Other things to prevent contamination during the fruiting stage are to keep the temperature lower and maintain adequate airflow.

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Got questions? Not sure where to start?

We're always here to help. If you'd rather dig in yourself, our Learning Centre is packed with guides on inoculation, fruiting, and troubleshooting.

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